Hi Len; I was having exactly the same problem with my crystals, but when we grow the crystals in presence of increasing concentration of Glycerol and MPD starting from 0.5 to 10%. The crystal doesn't appear after 3% of Glycerol or MPD but the one which appear in 2.5 to 3 % were much resistant to cracking than the original crystals.
Good luck Bashir On Wed, October 26, 2011 18:46, Leonard Thomas wrote: > Hi All, > > I have run into a very sensitive crystals system when it comes to cryo > protecting them. I have run through the usual suspects and trays are > going to be setup with a cryo protectant as part of crystallization > cocktail. The one problem that seems to be occurring is that the > crystals crack as soon as they are transfered out of the original > drop. I am running out of ideas and really would love some new ones. > > Thanks in advance. > > Len > > Leonard Thomas Ph.D. > Macromolecular Crystallography Laboratory Manager > University of Oklahoma > Department of Chemistry and Biochemistry > Stephenson Life Sciences Research Center > 101 Stephenson Parkway > Norman, OK 73019-5251 > > [email protected] > http://barlywine.chem.ou.edu > Office: (405)325-1126 > Lab: (405)325-7571 > -- Muhammad Bashir Khan ************************************************** Department for Structural and Computational Biology Max F. Perutz Laboratories University of Vienna Campus Vienna Biocenter 5 A-1030 Vienna Austria Austria Phone: +43(1)427752224 Fax: +43(1)42779522
