A good number of things to try. Just a little more info that was
asked for. The crystals are grown in Peg 3350 over a range of pH
values using Bis-Tris Propane. The are coming out of 2 different salt
conditions. My feeling is it is an osmolality problem though I also
observed cracking when going into a separately made well solution. I
will look it trying a number of suggestions given.
Cheers,
Len
Leonard Thomas Ph.D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
Department of Chemistry and Biochemistry
Stephenson Life Sciences Research Center
101 Stephenson Parkway
Norman, OK 73019-5251
[email protected]
http://barlywine.chem.ou.edu
Office: (405)325-1126
Lab: (405)325-7571
On Oct 26, 2011, at 12:54 PM, Muhammed bashir Khan wrote:
Hi Len;
I was having exactly the same problem with my crystals, but when we
grow
the crystals in presence of increasing concentration of Glycerol and
MPD
starting from 0.5 to 10%. The crystal doesn't appear after 3% of
Glycerol
or MPD but the one which appear in 2.5 to 3 % were much resistant to
cracking than the original crystals.
Good luck
Bashir
On Wed, October 26, 2011 18:46, Leonard Thomas wrote:
Hi All,
I have run into a very sensitive crystals system when it comes to
cryo
protecting them. I have run through the usual suspects and trays are
going to be setup with a cryo protectant as part of crystallization
cocktail. The one problem that seems to be occurring is that the
crystals crack as soon as they are transfered out of the original
drop. I am running out of ideas and really would love some new ones.
Thanks in advance.
Len
Leonard Thomas Ph.D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
Department of Chemistry and Biochemistry
Stephenson Life Sciences Research Center
101 Stephenson Parkway
Norman, OK 73019-5251
[email protected]
http://barlywine.chem.ou.edu
Office: (405)325-1126
Lab: (405)325-7571
--
Muhammad Bashir Khan
**************************************************
Department for Structural and Computational Biology
Max F. Perutz Laboratories
University of Vienna
Campus Vienna Biocenter 5
A-1030 Vienna
Austria
Austria
Phone: +43(1)427752224
Fax: +43(1)42779522
