Dear Jose, I have an iTC200 in my lab and have experience with a VP-ITC. The major problem with the iTC200 is that GE has not increased the sensitivity of the instrument. Thus, you must use higher concentrations in both the cell and syringe to see measurable heats. The syringe on the iTC200 holds 40 uLs. The other issue is the quality of the products from GE. I have replaced 2 boards on the instrument and replaced the washing module. GE manufacturing is going down hill and there equipment is really expensive.
I would really consider demoing TA instruments. I have done this for purchase of a capillary DSC instruments. TA instruments quoted me $75,000 for their cap DSC. The similar cap DSC by GE was quoted for $150,000. We are purchasing the cap DSC from TA instruments. In retrospect, I should have done the same thing for the ITC instruments. Cheers, Scott ************************************************ Scott T. R. Walsh, Ph.D. Assistant Professor University of Maryland College Park Dept of Cell Biology and Molecular Genetics Institute for Bioscience and Biotechnology Research Rm 3127E SG II 9600 Gudelsky Drive Rockville, MD 20850 email: [email protected] phone: (240) 314-6478 fax: (240) 314-6255 On Jan 17, 2012, at 11:02 AM, Jose Artur Brito wrote: > Dear All, > sorry for this off-topic questions but I would like to have some > feed-back from you on Isothermal Titration Calorimetry (ITC) equipments. > We have a very nice quotation for an iTC200 from GE Healthcare. We > wanted this one because it uses ~200uL sample per measurement (really > nice when your dealing with "precious" samples, ie., proteins with low > expression yields). However, I was told that, although consuming much > less sample, is not as good (sensitivity, mixing issues, bubbles, ...), > as the VP-ITC (it uses ~1.4mL per measurement, seven times more than the > iTC200). > Does anyone has experience with these two equipments? Would you prefer > one over the other (please state your reasons)? Would you suggest > another equipment/brand for the ITC (like the NanoITC from TA Instruments)? > Thanks in advance, > Jose Artur Brito > > -- > ************************************************ > * José Artur Brito, PhD * > * * > * Post-Doctoral Fellow * > * Membrane Protein Crystallography Lab * > * Instituto de Tecnologia Química e Biológica * > * Oeiras - Portugal * > * * > * Tel.: +351.21.446.97.61 * > * Fax: +351.21.443.36.44 * > * * > * E-mail: [email protected] * > * URL: http://mx.itqb.unl.pt * > ************************************************
