We bought a NanoITC from TA instruments last year and I can't recommend it enough. We are able to get excellent binding curves with the low volume cell/syringe (200uL/50uL, respectively, which actually means 350uL/120uL in order to load both without bubbles) using the same concentrations of protein as we used to use in a MicroCal VP-ITC. The control/analysis software is much easier to use (although the graphing capabilities could be improved) and the technical support has thus far has been excellent. Also, as others have said it's a steal compared with the equivalent GE machine (both in terms of purchase cost and cost of maintenance contracts).
Stephen On 17 January 2012 16:02, Jose Artur Brito <[email protected]> wrote: > Dear All, > sorry for this off-topic questions but I would like to have some feed-back > from you on Isothermal Titration Calorimetry (ITC) equipments. > We have a very nice quotation for an iTC200 from GE Healthcare. We wanted > this one because it uses ~200uL sample per measurement (really nice when > your dealing with "precious" samples, ie., proteins with low expression > yields). However, I was told that, although consuming much less sample, is > not as good (sensitivity, mixing issues, bubbles, ...), as the VP-ITC (it > uses ~1.4mL per measurement, seven times more than the iTC200). > Does anyone has experience with these two equipments? Would you prefer one > over the other (please state your reasons)? Would you suggest another > equipment/brand for the ITC (like the NanoITC from TA Instruments)? > Thanks in advance, > Jose Artur Brito > > -- > ************************************************ > * José Artur Brito, PhD * > * * > * Post-Doctoral Fellow * > * Membrane Protein Crystallography Lab * > * Instituto de Tecnologia Química e Biológica * > * Oeiras - Portugal * > * * > * Tel.: +351.21.446.97.61 * > * Fax: +351.21.443.36.44 * > * * > * E-mail: [email protected] * > * URL: http://mx.itqb.unl.pt * > ************************************************ -- Dr Stephen Graham 1851 Research Fellow Cambridge Institute for Medical Research Wellcome Trust/MRC Building Addenbrooke's Hospital, Hills Road Cambridge, CB2 0XY, UK Phone: +44 1223 762 638
