Hi Rajesh, If I understand correctly, the phospho groups become disconnected from either the serine or threonine. If this is the case, you can use the SEP monomer for phosphoserine and TPO monomer for phosphothreonine. (replace the amino acid residues with these)
These are built into ccp4 and coot Hope this helps Joel _________________________________ Joel Tyndall, PhD Senior Lecturer in Medicinal Chemistry National School of Pharmacy University of Otago PO Box 56 Dunedin 9054 New Zealand Skype: jtyndall http://www.researcherid.com/rid/C-2803-2008 Pukeka Matua Te Kura Taiwhanga Putaiao Te Whare Wananga o Otago Pouaka Poutapeta 56 Otepoti 9054 Aotearoa Ph / Waea +64 3 4797293 Fax / Waeawhakaahua +64 3 4797034 From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Rajesh kumar Sent: Thursday, 22 March 2012 12:00 p.m. To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] refining phosphorylated residues Dear All, I have a structure of a protein and peptide complex, in which peptide has modified residues ( phosphoserine and phosphothreonine). During refinement these both gets disconnected with adjacent residues and its hard to connect them. Could you please suggest me some options. Thanks Rajesh