Dear All, Thanks for the suggestions. I will work on them.I had not worked on the modified residues, so thanks for all the valuable suggestions. RegardsRajesh
Date: Thu, 22 Mar 2012 10:15:55 +0000 From: [email protected] Subject: Re: [ccp4bb] refining phosphorylated residues To: [email protected] In new version (it should be in ccp4 6.2.0, if not then it will come ccp4 6.3, otherwise you can take it from York's web site: ) TPO as well as SEP are peptides. Break in coot may be due to misinterpretation of SEP or TPO as peptide in coot and it may be because of older version of the dictionary. Do the distances, angles between neighbouring residues make sence? If not then refinement also had problems regardsGarib On 22 Mar 2012, at 00:39, Joel Tyndall wrote:As a follow up question the bulletin board, why is SEP a peptide (L-peptide) and TPO not (non-polymer)? Joel _________________________________Joel Tyndall, PhD Senior Lecturer in Medicinal Chemistry National School of Pharmacy University of Otago PO Box 56 Dunedin 9054 New Zealand Skype: jtyndall http://www.researcherid.com/rid/C-2803-2008Pukeka Matua Te Kura Taiwhanga Putaiao Te Whare Wananga o Otago Pouaka Poutapeta 56 Otepoti 9054 Aotearoa Ph / Waea +64 3 4797293 Fax / Waeawhakaahua +64 3 4797034 From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Rajesh kumar Sent: Thursday, 22 March 2012 12:00 p.m. To: [email protected] Subject: [ccp4bb] refining phosphorylated residues Dear All, I have a structure of a protein and peptide complex, in which peptide has modified residues ( phosphoserine and phosphothreonine). During refinement these both gets disconnected with adjacent residues and its hard to connect them.Could you please suggest me some options. ThanksRajesh Garib N Murshudov Structural Studies DivisionMRC Laboratory of Molecular BiologyHills Road Cambridge CB2 0QH UK Email: [email protected] Web http://www.mrc-lmb.cam.ac.uk
