Hi Joel, thanks for the reply. It's not phospho group but the whole residue get disconnected. In coot if I do refinement they get disconnected.
Thanks Rajesh Sent from my iPhone On Mar 21, 2012, at 7:38 PM, "Joel Tyndall" <[email protected]> wrote: > Hi Rajesh, > > If I understand correctly, the phospho groups become disconnected from either > the serine or threonine. If this is the case, you can use the SEP monomer for > phosphoserine and TPO monomer for phosphothreonine. (replace the amino acid > residues with these) > > These are built into ccp4 and coot > > Hope this helps > > Joel > > _________________________________ > Joel Tyndall, PhD > > Senior Lecturer in Medicinal Chemistry > National School of Pharmacy > University of Otago > PO Box 56 Dunedin 9054 > New Zealand > Skype: jtyndall > http://www.researcherid.com/rid/C-2803-2008 > Pukeka Matua > Te Kura Taiwhanga Putaiao > Te Whare Wananga o Otago > Pouaka Poutapeta 56 Otepoti 9054 > Aotearoa > > Ph / Waea +64 3 4797293 > Fax / Waeawhakaahua +64 3 4797034 > > From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Rajesh > kumar > Sent: Thursday, 22 March 2012 12:00 p.m. > To: [email protected] > Subject: [ccp4bb] refining phosphorylated residues > > Dear All, > > I have a structure of a protein and peptide complex, in which peptide has > modified residues ( phosphoserine and phosphothreonine). > During refinement these both gets disconnected with adjacent residues and > its hard to connect them. > Could you please suggest me some options. > > Thanks > Rajesh
