Hello, I am developing an undergraduate biochemistry lab class and would like to incorporate experiments with membrane proteins. Does anyone have suggestions on membrane proteins that are relatively easy to express, purify, and assay? Bonus points for crystallizable! At the moment, my leading candidate is aquaporin AqpZ from E. coli. I am planning to express the membrane protein as a GFP fusion so students can easily follow it through the course of the labs.
Thank you, Ho Ho Leung Ng University of Hawaii at Manoa Assistant Professor, Department of Chemistry h...@hawaii.edu