Two additional suggestions: 1. Photosynthetic Reaction Center from *R. sphaeroides* might be up your alley. Similar to bacteriorhodopsin, no need for a GFP tag on this one as it's colored. See the following publications:
Wallace et al. "Monoolein Lipid phases as incorporation and inrichment materials for membrane protein crystallization" PLoS One. Aug 31 2011. Kors et al. "Effects of impurities on membrane-protein crystalllzation in different systems" Acta Cryst D Biol Crystallogr. Oct 2009. p1062-73. 2. The beta barrel transmembrane domain of intimin would work as well. Expresses in standard BL21 DE3 *E. coli* cells, 3 step purification, crystallizes readily in LCP, and is stable for months at a time at 4 degrees (not colored though): Fairman et al. "Crystal Structures of the outer membrane domain of intimin and invasin from enterohemorrhagic *E. coli* and enteropathogenic *Y. pseudotuberculosis*". Structure. Jul 3 2012. p 1233-43. Cheers, Jim On Tue, Sep 11, 2012 at 2:18 PM, Ho Leung Ng <[email protected]> wrote: > Hello, > > I am developing an undergraduate biochemistry lab class and > would like to incorporate experiments with membrane proteins. Does > anyone have suggestions on membrane proteins that are relatively easy > to express, purify, and assay? Bonus points for crystallizable! At the > moment, my leading candidate is aquaporin AqpZ from E. coli. I am > planning to express the membrane protein as a GFP fusion so students > can easily follow it through the course of the labs. > > > Thank you, > Ho > > Ho Leung Ng > University of Hawaii at Manoa > Assistant Professor, Department of Chemistry > [email protected] > -- Jim Fairman, Ph D. Crystal Core Leader I Emerald BioStructures <http://www.emeraldbiostructures.com/> Tel: 206-780-8914 Cell: 240-479-6575 E-mail: [email protected] [email protected]
