The multidrug efflux transporter AcrB could be suitable including crystallization (both recombinant with His-tag and endogenous). Extracts, solubilizes, purifies and crystallizes in DDM, so it is fairly inexpensive to repeat in bulk compared to other detergents. Assays might be a bit complicated.
And it is impressive in architecture too, forming a trimer with 12 TM in each monomer, with 70A span in the periplasm and 50A in the inner membrane and very little in the cytoplasm. Thanks, Debanu. -----Original Message----- From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Ho Leung Ng Sent: Tuesday, September 11, 2012 2:19 PM To: [email protected] Subject: [ccp4bb] the lysozyme of membrane proteins? Hello, I am developing an undergraduate biochemistry lab class and would like to incorporate experiments with membrane proteins. Does anyone have suggestions on membrane proteins that are relatively easy to express, purify, and assay? Bonus points for crystallizable! At the moment, my leading candidate is aquaporin AqpZ from E. coli. I am planning to express the membrane protein as a GFP fusion so students can easily follow it through the course of the labs. Thank you, Ho Ho Leung Ng University of Hawaii at Manoa Assistant Professor, Department of Chemistry [email protected]
