[1] In addition to using lower resolution data, you should check a variety of RMSDs as well. In fact constructing an RMSD / high resolution contour plot would be beneficial to determine the optimum set of parameters. (That is if you're using PHASER).
[2] You say you suspect two molecules per asymmetric unit. Are you fortunate enough to have NCS that's not aligned with the crystal axes? (Have you checked the self rotation patterson?). If there's significant off origin peaks you can (a) try to model the dimer and use that for the search or (b) use the locked rotation function in molrep. [3] Any heavy atoms in the structure (Zn, FeS, etc)? F On Oct 1, 2012, at 12:26 PM, RHYS GRINTER wrote: > Just for background, the datasets I have are 2 to 2.7 angstroms with pretty > nice stats. The space group is most likely C2221 with two molecules per ASU > (giving around 58% solvent).
