-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Rhys,
with good 2.0A data you could try S-SAD, or MR-SAD. For the latter I recommend autorickshaw (http://www.embl-hamburg.de/Auto-Rickshaw/). Best, Tim On 10/01/2012 09:26 PM, RHYS GRINTER wrote: > Hi All, > > I'm currently working on solving the structure of a protein by > molecular replacement. The protein is around 30kDa and likely has a > two beta-prism domains, linked by a long curved two stranded sheet > based on the structure of an analogue. There are also a number of > other structures which represent a single homologous beta-prism > domain. I've tried to find MR solution using the analogue and > various truncation/AA substitution models based on it with no > success. I've also tried single domain ensembles of the other > homologous structures, also with no success. I think the problem is > the overall sequence homology is quite low between my protein and > the available structures (35% for the analogue and around 20% for > the other models. > > I was curious as to how someone with more experience would tackle > this problem. > > Just for background, the datasets I have are 2 to 2.7 angstroms > with pretty nice stats. The space group is most likely C2221 with > two molecules per ASU (giving around 58% solvent). > > Thanks, > > Rhys Grinter PhD Candidate University of Glasgow - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFQapxiUxlJ7aRr7hoRAnljAKCSi4mxkp0BJ/FvrLQNJszCtLtToACgya1H SN6qckg9//iN2KrNU/oxMXE= =ZOpN -----END PGP SIGNATURE-----
