Couldn't you just run reducing/non-reducing SDS-PAGE lanes and see the difference?
JPK On Wed, Feb 6, 2013 at 11:10 AM, Yuri Pompeu <[email protected]> wrote: > Dear All, > I am trying to probe the existence of a disulfide bond on the surface of > my protein. > I have attempted Ellman´s and my results were not as clear as I would have > hoped for. > I am not a sulfur/cysteine chemist and would appreciate the advice on what > experiments to try! > Thanks a bunch > YAP > -- ******************************************* Jacob Pearson Keller, PhD Postdoctoral Associate HHMI Janelia Farms Research Campus email: [email protected] *******************************************
