Perhaps you should have a look at
http://193.146.160.29/gtb/sod/usu/$UBUG/repositorio/10320076_Hawkins.pdf.
and at "An introduction to methods for analyzing thiols and disulfides:
Reactions, reagents, and practical considerations. Rosa E. Hansen 1,
Jakob R. Winther Analytical Biochemistry 394 (2009) 147–158.
HTH,
Nadir Mrabet
Pr. Nadir T. Mrabet
Structural & Molecular Biochemistry
N-gere - INSERM U-954
University of Lorraine, Nancy
School of Sciences and Technologies
& School of Medicine
9, Avenue de la Foret de Haye, BP 184
54505 Vandoeuvre-les-Nancy Cedex
France
Phone: +33 (0)3.83.68.32.73
Fax: +33 (0)3.83.68.32.79
E-mail: Nadir.Mrabet <at> univ-lorraine.fr
On 07/02/2013 04:17, Dr. Anthony Addlagatta wrote:
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Dear Yuri,
If you have access to mass spec, this should be a straight forward experiment.
Find the
reference here.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2291582/pdf/v010p00017.pdf
What was the result in the Ellman´s reaction? Unless you have other reactive
cysteines
in your protein protein, you should not see any color if the pair of cysteines
on
surface form disulfide.
Anthony
-----------------------------------------------------
Dr. Anthony Addlagatta
Center for Chemical Biology
Indian Institute of Chemical Technology [IICT]
Tarnaka, Hyderabad
AP-500 607, INDIA
Tel:91-40-27191812
Web: https://sites.google.com/site/chembioliict/home/dr-anthony-addlagatta-1
---------- Original Message -----------
From: Yuri Pompeu <[email protected]>
To: [email protected]
Sent: Wed, 6 Feb 2013 16:10:35 +0000
Subject: [ccp4bb] Off Topic- Cystine Detection
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free of known security risks.
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Dear All,
I am trying to probe the existence of a disulfide bond on the surface of my
protein.
I have attempted Ellman´s and my results were not as clear as I would have
hoped for.
I am not a sulfur/cysteine chemist and would appreciate the advice on what
experiments
to try!
Thanks a bunch
YAP
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