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Dear Yuri, If you have access to mass spec, this should be a straight forward experiment. Find the reference here. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2291582/pdf/v010p00017.pdf What was the result in the Ellman´s reaction? Unless you have other reactive cysteines in your protein protein, you should not see any color if the pair of cysteines on surface form disulfide. Anthony ----------------------------------------------------- Dr. Anthony Addlagatta Center for Chemical Biology Indian Institute of Chemical Technology [IICT] Tarnaka, Hyderabad AP-500 607, INDIA Tel:91-40-27191812 Web: https://sites.google.com/site/chembioliict/home/dr-anthony-addlagatta-1 ---------- Original Message ----------- From: Yuri Pompeu <yuri.pom...@ufl.edu> To: CCP4BB@JISCMAIL.AC.UK Sent: Wed, 6 Feb 2013 16:10:35 +0000 Subject: [ccp4bb] Off Topic- Cystine Detection > *********************** > This message has been scanned by the InterScan for CSC SSM at IICT and found > to be free of known security risks. > *********************** > > Dear All, > I am trying to probe the existence of a disulfide bond on the surface of my > protein. > I have attempted Ellman´s and my results were not as clear as I would have > hoped for. > I am not a sulfur/cysteine chemist and would appreciate the advice on what > experiments to try! > Thanks a bunch > YAP ------- End of Original Message ------- This Mail Scanned by ClamAV and Spammassassin
