We add 0.02% Azide to all the water used for washing in our Phoenix, and that seems to help.
Janet Newman Principal Scientist / Director, Collaborative Crystallisation Centre CSIRO Material Science and Engineering 343 Royal Parade Parkville. VIC. 3052 Australia Tel +613 9662 7326 Email [email protected] ________________________________________ From: CCP4 bulletin board [[email protected]] On Behalf Of Viswanathan Chandrasekaran [[email protected]] Sent: 09 March 2013 10:24 To: [email protected] Subject: [ccp4bb] Off-topic: Fungal growth in robot trays Dear All: I would like some advice on getting rid of persistent fungal growth in 96-well sitting drop crystal plates that were set up using a Phoenix robot. 24-well sitting drop trays prepared by hand don’t have this problem. Washing the robot with 0.5% bleach followed by plenty of water had no effect. Is adding sodium azide directly to commercial screen hotels (or the protein sample) a good idea? If so, how much should I add? Other suggestions are welcome. I will post a summary of all replies. Thank you. Best, Vish
