Dear All: Thank you for your responses. Here is a summary of suggested fixes:
1. Cleaning the supply carboy and lines with bleach and flushing thoroughly with DD water afterwards 2. Adding 0.02% sodium azide to the protein 3. Adding 0.02% azide to commercial screens 4. Adding 0.02% azide to the water used for washing 5. Using fresh screens and storing them at low temperatures (4 or 12 degree C) 6. Manually dispensing the reservoir solution using a multi-channel pipette 7. Using a Mosquito robot (it uses fresh needles each time) Best, Vish From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Viswanathan Chandrasekaran Sent: Friday, March 08, 2013 4:24 PM To: [email protected] Subject: [ccp4bb] Off-topic: Fungal growth in robot trays Dear All: I would like some advice on getting rid of persistent fungal growth in 96-well sitting drop crystal plates that were set up using a Phoenix robot. 24-well sitting drop trays prepared by hand don't have this problem. Washing the robot with 0.5% bleach followed by plenty of water had no effect. Is adding sodium azide directly to commercial screen hotels (or the protein sample) a good idea? If so, how much should I add? Other suggestions are welcome. I will post a summary of all replies. Thank you. Best, Vish
