8. Empty the carboy and fill the supply lines and wash stations with 20% ethanol at the end of the day. (Requires decontamination in this case)
Best regards, Dmitry On 2013-03-13, at 10:04 PM, Viswanathan Chandrasekaran <[email protected]> wrote: > Dear All: > Thank you for your responses. Here is a summary of suggested fixes: > 1. Cleaning the supply carboy and lines with bleach and flushing > thoroughly with DD water afterwards > 2. Adding 0.02% sodium azide to the protein > 3. Adding 0.02% azide to commercial screens > 4. Adding 0.02% azide to the water used for washing > 5. Using fresh screens and storing them at low temperatures (4 or 12 > degree C) > 6. Manually dispensing the reservoir solution using a multi-channel > pipette > 7. Using a Mosquito robot (it uses fresh needles each time) > Best, > Vish > > From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of > Viswanathan Chandrasekaran > Sent: Friday, March 08, 2013 4:24 PM > To: [email protected] > Subject: [ccp4bb] Off-topic: Fungal growth in robot trays > > Dear All: > I would like some advice on getting rid of persistent fungal growth in > 96-well sitting drop crystal plates that were set up using a Phoenix robot. > 24-well sitting drop trays prepared by hand don’t have this problem. Washing > the robot with 0.5% bleach followed by plenty of water had no effect. Is > adding sodium azide directly to commercial screen hotels (or the protein > sample) a good idea? If so, how much should I add? Other suggestions are > welcome. > I will post a summary of all replies. > Thank you. > Best, > Vish
