Dear All, I crystallized a complex of two proteins and got x-ray data. However I dont know if I have got complex or just one of the protein has crystallized. Can I check it by mathew's coefficient? If yes how? One of my protein is 30 kDa nad other one is 12 kDa.
Actually I calculated Mathew's coeffient (based on Mol. wt of complex 42000 Da), and results were like that Cell Volume = 993567 Nmol/asym Mathews coeff %solvent 1 3.94 68 2 1.97 37 3 1.3 6 Can any one please explain what does it suggest????? Thanks Abbas
