Looks like 1 or 2 protein units per ASU. Two seems most likely, but I
have had a bunch of crystals with 67% solvent, too, so 1 is not out of
the question if less probable. So try both with your favorite MR method
and see what happens. Crystal packing in the MR solution will tell all.
_______________________________________
Roger S. Rowlett
Gordon & Dorothy Kline Professor
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346
tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: rrowl...@colgate.edu
On 12/4/2013 5:06 PM, abbas maqbool wrote:
Dear All,
I crystallized a complex of two proteins and got x-ray data. However I
dont know if I have got complex or just one of the protein has
crystallized. Can I check it by mathew's coefficient? If yes how? One
of my protein is 30 kDa nad other one is 12 kDa.
Actually I calculated Mathew's coeffient (based on Mol. wt of complex
42000 Da), and results were like that
Cell Volume = 993567
Nmol/asym Mathews coeff %solvent
1 3.94 68
21.9737
31.36
Can any one please explain what does it suggest?????
Thanks
Abbas
