If either of the two protein structures has been determined/deposited, I would check if your unit cell matched one of them. Kris F. Tesh, Ph. D. Department of Biology and Biochemistry University of Houston
________________________________ From: "Tanner, John J." <[email protected]> To: [email protected] Sent: Wednesday, December 4, 2013 4:29 PM Subject: Re: [ccp4bb] Can Mathew's coefficient tell about a complex First of all, there are two Ts and no apostrophe in Matthews. The method is based on the following paper, which is worth reading. It is one of the first examples, possibly the first, of structural bioinformatics. J Mol Biol. 1968 Apr 28;33(2):491-7. Solvent content of protein crystals. Matthews BW. http://www.ncbi.nlm.nih.gov/pubmed/5700707 Secondly, you might run a gel on your crystals. John J. Tanner Professor of Biochemistry and Chemistry University of Missouri-Columbia 125 Chemistry Building Columbia, MO 65211 Phone: 573-884-1280 Fax: 573-882-2754 Email: [email protected] http://faculty.missouri.edu/~tannerjj/tannergroup/tanner.html On Dec 4, 2013, at 4:06 PM, abbas maqbool <[email protected]> wrote: Dear All, >I crystallized a complex of two proteins and got x-ray data. However I dont >know if I have got complex or just one of the protein has crystallized. Can I >check it by mathew's coefficient? If yes how? One of my protein is 30 kDa nad >other one is 12 kDa. > >Actually I calculated Mathew's coeffient (based on Mol. wt of complex 42000 >Da), and results were like that > > > > >Cell Volume = 993567 >Nmol/asym Mathews coeff %solvent >1 3.94 68 >2 1.97 37 >3 1.3 6 > > > > >Can any one please explain what does it suggest????? > > >Thanks >Abbas > > >
