If you want to know what in your crystal, run SDS-PAGE of your crystal.
On Thu, Dec 5, 2013 at 6:39 AM, Kris Tesh <kris.t...@att.net> wrote: > If either of the two protein structures has been determined/deposited, I > would check if your unit cell matched one of them. > > Kris F. Tesh, Ph. D. > Department of Biology and Biochemistry > University of Houston > > ------------------------------ > *From:* "Tanner, John J." <tanne...@missouri.edu> > *To:* CCP4BB@JISCMAIL.AC.UK > *Sent:* Wednesday, December 4, 2013 4:29 PM > *Subject:* Re: [ccp4bb] Can Mathew's coefficient tell about a complex > > First of all, there are two Ts and no apostrophe in Matthews. The > method is based on the following paper, which is worth reading. It is one > of the first examples, possibly the first, of structural bioinformatics. > > J Mol Biol. 1968 Apr 28;33(2):491-7. > Solvent content of protein crystals. > Matthews BW. > http://www.ncbi.nlm.nih.gov/pubmed/5700707 > > Secondly, you might run a gel on your crystals. > > > John J. Tanner > Professor of Biochemistry and Chemistry > University of Missouri-Columbia > 125 Chemistry Building > Columbia, MO 65211 > Phone: 573-884-1280 > Fax: 573-882-2754 > Email: tanne...@missouri.edu > http://faculty.missouri.edu/~tannerjj/tannergroup/tanner.html > > On Dec 4, 2013, at 4:06 PM, abbas maqbool <abbas_maqb...@yahoo.com> > wrote: > > Dear All, > I crystallized a complex of two proteins and got x-ray data. However I > dont know if I have got complex or just one of the protein has > crystallized. Can I check it by mathew's coefficient? If yes how? One of my > protein is 30 kDa nad other one is 12 kDa. > Actually I calculated Mathew's coeffient (based on Mol. wt of complex > 42000 Da), and results were like that > > > Cell Volume = 993567 > Nmol/asym Mathews coeff %solvent > 1 3.94 68 > 2 1.97 37 > 3 1.3 6 > > > Can any one please explain what does it suggest????? > > Thanks > Abbas > > > > >
