Dear Careina – Orthogonal crystallization methods can be of great utility when optimizing crystallization conditions, since they offer a different sampling of the crystallization space. Orthogonal methods include: liquid-liquid diffusion, microbatch and dialysis.
Liquid-liquid diffusion is known to be a productive crystallization method and a recent paper from the Sundberg lab demonstrated that crystallization by liquid-liquid diffusion could improve both the size and diffraction quality of crystals of an endo-b-N-acetylglucosaminidase, EndoS, from Streptococcus pyogenes. The paper can be found here: http://scripts.iucr.org/cgi-bin/paper?nj5178 Description of the crystallization device can be found here: http://scripts.iucr.org/cgi-bin/paper?S1744309111024456 and http://www.microlytic.com/crystal-former Using microseed matrix screening could also improve your crystal quality. You can find information on the approach here: http://pubs.acs.org/doi/abs/10.1021/cg2001442?journalCode=cgdefu Best regards, Morten -------- Original message -------- Subject:[SURESPAM] [ccp4bb] small crystals From:Careina Edgooms <careinaedgo...@yahoo.com<mailto:careinaedgo...@yahoo.com>> To:CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Cc: Hi all Any advice on how to get bigger crystals from conditions that give showers of tiny crystals? I am getting small pretty looking individual crystals but they are too small and they don't seem to grow. In fact, in some instances if left for a couple of days they actually dissolve. I have fiddled around with mother liquor volume, protein concentration as well as drop volume (I am using hanging drop method) but none seem to make any difference and I always get the same tiny crystals. I think I might try microseeding but I haven't tried that yet. Any suggestions or tricks would be welcome Careina. -- TotalCare Message Security: Click below to verify authenticity http://www.exchangedefender.com/verify.asp?id=rB5EYXWs004083&from=m...@microlytic.com
