Dear Jens, Careina this is not really an *advantage*, but rather a *convenience*. You can still use big crystals if you'd like to, but as they usually never survive more than one shot (few femtosec), you'd need a lot of these bigger crystals to collect a full data.
And yes, I would also highly recommend XFELs! Cheers, Leo On Dec 10, 2013, at 4:36 AM, Jens Kaiser <[email protected]> wrote: > Careina, > If your target is interesting enough, try to reproduce the small > crystals in batch and apply for FELS time. Small crystals are actually > an advantage there. > > Cheers, > > Jens > > > On Wed, 2013-12-04 at 21:41 -0800, Careina Edgooms wrote: >> Hi all >> >> >> Any advice on how to get bigger crystals from conditions that give >> showers of tiny crystals? I am getting small pretty looking individual >> crystals but they are too small and they don't seem to grow. In fact, >> in some instances if left for a couple of days they actually dissolve. >> I have fiddled around with mother liquor volume, protein concentration >> as well as drop volume (I am using hanging drop method) but none seem >> to make any difference and I always get the same tiny crystals. I >> think I might try microseeding but I haven't tried that yet. >> >> >> Any suggestions or tricks would be welcome >> Careina.
