Hi,
can you give a bit more information...
Can you concentrate the protein easily to a higher concentration,
let's say 2-3 times from what you have now, without precipitation?
What is the buffer of your protein stock solution at the moment?
At what temperature and what precipitant are you using?
- Jeroen -
showers of crystals
-------- Original message --------
Subject:[SURESPAM] [ccp4bb] small crystals
From:Careina Edgooms < careinaedgo...@yahoo.com>
To: CCP4BB@JISCMAIL.AC.UK
Cc:
Hi all
Any advice on how to get bigger crystals from
conditions that give showers of tiny crystals? I am
getting small pretty looking individual crystals but
they are too small and they don't seem to grow. In
fact, in some instances if left for a couple of days
they actually dissolve. I have fiddled around with
mother liquor volume, protein concentration as well as
drop volume (I am using hanging drop method) but none
seem to make any difference and I always get the same
tiny crystals. I think I might try microseeding but I
haven't tried that yet.
Any suggestions or tricks would be welcome
Careina.
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