Yes, Tim is right… for now… in few years, with the E-XFEL, we'll get to much less sample, and much more time available. But that's in few years.
Leo On Dec 10, 2013, at 10:01 AM, Tim Gruene <t...@shelx.uni-ac.gwdg.de> wrote: > Dear Careina, > > you can also apply for beamtime at PETRA-III. You get away with the same > size crystals but only require a nano liter drop rather than a few ml of > your sample. And you probably get beamtime much quicker because all the > equipment is installed and collecting a data set takes very short time. > This was demonstrated at the ECM in Warwick this year, so no need for > FEL (at least for structure determination). > > Best, > Tim > > On 12/10/2013 04:36 AM, Jens Kaiser wrote: >> Careina, >> If your target is interesting enough, try to reproduce the small >> crystals in batch and apply for FELS time. Small crystals are actually >> an advantage there. >> >> Cheers, >> >> Jens >> >> >> On Wed, 2013-12-04 at 21:41 -0800, Careina Edgooms wrote: >>> Hi all >>> >>> >>> Any advice on how to get bigger crystals from conditions that give >>> showers of tiny crystals? I am getting small pretty looking individual >>> crystals but they are too small and they don't seem to grow. In fact, >>> in some instances if left for a couple of days they actually dissolve. >>> I have fiddled around with mother liquor volume, protein concentration >>> as well as drop volume (I am using hanging drop method) but none seem >>> to make any difference and I always get the same tiny crystals. I >>> think I might try microseeding but I haven't tried that yet. >>> >>> >>> Any suggestions or tricks would be welcome >>> Careina. >> > > -- > Dr Tim Gruene > Institut fuer anorganische Chemie > Tammannstr. 4 > D-37077 Goettingen > > GPG Key ID = A46BEE1A >
