Step1: visit Protparam tool and C&P your sequence, scroll down until you find the extinction coefficient part. If OD280 is not close to 1 then make sure to take that into account in your chromatogram say it is 0.7 step2: look at your mAU's If your peak is 1000 mAU then you have 0.7 mg/ml in that fraction Step3: you are pretty lazy google for it
Jürgen On Jan 15, 2014, at 10:09 AM, Karel Chaz <karel.c...@gmail.com<mailto:karel.c...@gmail.com>> wrote: Dear all, A question for the biochemistry-inclined folks in the bb; how do I calculate protein concentration of chromatography fractions, starting from Abs280 from the UV monitor? I know I could figure it out myself if I really tried, but why bother when I have access to so many brilliant minds.... Thanks to all, K ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
