On 1/15/14, 9:34 AM, Matthew Franklin wrote:
- I usually approximate the peak as a triangle, so that the total peak area is 1/2 height (absorbance maximum) x base (the number of ml in your pool)
GE's UNICORN for AKTAs (and probably the new Biorad FPLC software) integrates and tells you the area under whatever peak. Baselines and peak boundaries are user adjustable.

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