I don't think it possible for protein purification purpose. There would be no linear correlation between A280 and the protein concentration.
For pure protein analysis, as for minor amount protein used, it is possible. Acoot ________________________________ From: Karel Chaz <karel.c...@gmail.com> To: CCP4BB@JISCMAIL.AC.UK Sent: Wednesday, 15 January 2014 11:09 PM Subject: [ccp4bb] Protein concentration form chromatograms Dear all, A question for the biochemistry-inclined folks in the bb; how do I calculate protein concentration of chromatography fractions, starting from Abs280 from the UV monitor? I know I could figure it out myself if I really tried, but why bother when I have access to so many brilliant minds.... Thanks to all, K
