I am not sure, but probably path length not 1 cm in a detector cuvette .
You must refer to your HPLS system manual for this value
15.01.2014 19:09, Karel Chaz пишет:
Dear all,
A question for the biochemistry-inclined folks in the bb; how do I
calculate protein concentration of chromatography fractions, starting
from Abs280 from the UV monitor? I know I could figure it out myself
if I really tried, but why bother when I have access to so many
brilliant minds....
Thanks to all,
K
--
Eugene Osipov
Junior Research Scientist
Laboratory of Enzyme Engineering
A.N. Bach Institute of Biochemistry
Russian Academy of Sciences
Leninsky pr. 33, 119071 Moscow, Russia
e-mail: e.m.osi...@gmail.com
