Hi Phoebe -
"Cost-effective" may not be the applicable word here, but the
Microfluidizer works very well:
http://www.microfluidicscorp.com/index.php?option=com_content&view=article&id=19&Itemid=76
This gadget runs on house compressed air (don't try to use a compressed
air tank - you'll empty it in minutes). It's a bit noisy, but so is a
sonicator.
The Microfluidizer really shines with large volumes of lysate - like 1 L
and up. If you're only processing 100-200 mL at a time, I think
sonication is the best way to go.
Hope that helps,
Matt
On 2/4/14 11:49 AM, Phoebe A. Rice wrote:
Some time ago, there was a nice discussion of cost-effective, wimpy
protein-friendly ways to break open E. coli. We're thinking about
replacing an aging sonicator. If people have a favorite gizmo, could
they repeat that advice?
thank you,
Phoebe Rice
++++++++++++++++++++++++++++++++++++++++++
Phoebe A. Rice
Dept. of Biochemistry & Molecular Biology
The University of Chicago
773 834 1723; [email protected] <mailto:[email protected]>
http://bmb.bsd.uchicago.edu/Faculty_and_Research/
http://www.rsc.org/shop/books/2008/9780854042722.asp
--
Matthew Franklin, Ph. D.
Senior Scientist
New York Structural Biology Center
89 Convent Avenue, New York, NY 10027
(212) 939-0660 ext. 9374
