LOL. In any case, a French press is also not french, according to Wikipedia it was invented by Charles Stacy French of the Carnegie Institution of Washington (hence French press with capital F).
In any case, I agree, Emulsiflex, Constant Systems One-shot, Microfluidizer etc. is the way to go if you have some money. Mark J van Raaij Lab 20B Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/~mjvanraaij On 4 Feb 2014, at 18:09, Pascal Egea wrote: > Hi Phoebe, > > Another possibility is the Emulsiflex (from Avestin, in canada). Not a cheap > piece of equipment, but very sturdy and efficient to up to 200 mL of extract > (runs on house-air). Can deal with E coli (and even yeast if you have the > models with internal compressor). It comes in 3 sizes I think we have the > middle one (C-3) with a compressor. It is basically a french press but > without the inconvenient of being french (I am french myself). It is quite > gentle, and does not overheat samples as much as the sonicator. We make a lot > of membrane protein purifications and I have been working with this since my > post-doc > Hope this helps. > > Best regards, > > Pascal Egea > > > On Tue, Feb 4, 2014 at 8:49 AM, Phoebe A. Rice <pr...@uchicago.edu> wrote: > Some time ago, there was a nice discussion of cost-effective, wimpy > protein-friendly ways to break open E. coli. We're thinking about replacing > an aging sonicator. If people have a favorite gizmo, could they repeat that > advice? > thank you, > Phoebe Rice > > ++++++++++++++++++++++++++++++++++++++++++ > > Phoebe A. Rice > Dept. of Biochemistry & Molecular Biology > The University of Chicago > > 773 834 1723; pr...@uchicago.edu > http://bmb.bsd.uchicago.edu/Faculty_and_Research/ > > http://www.rsc.org/shop/books/2008/9780854042722.asp > > > > -- > Pascal F. Egea, PhD > Assistant Professor > UCLA, David Geffen School of Medicine > Department of Biological Chemistry > Boyer Hall room 356 > 611 Charles E Young Drive East > Los Angeles CA 90095 > office (310)-983-3515 > lab (310)-983-3516 > email pe...@mednet.ucla.edu
