I would also recommend the Emulsiflex line of cell disruptors from Avestin - it has been my favorite and most easily used instrument of cell destruction.
On Tue, Feb 4, 2014 at 9:17 AM, Mark J van Raaij <mjvanra...@cnb.csic.es>wrote: > LOL. > > In any case, a French press is also not french, according to Wikipedia it > was invented by Charles Stacy French of the Carnegie Institution of > Washington (hence French press with capital F). > > In any case, I agree, Emulsiflex, Constant Systems One-shot, > Microfluidizer etc. is the way to go if you have some money. > > Mark J van Raaij > Lab 20B > Dpto de Estructura de Macromoleculas > Centro Nacional de Biotecnologia - CSIC > c/Darwin 3 > E-28049 Madrid, Spain > tel. (+34) 91 585 4616 > http://www.cnb.csic.es/~mjvanraaij > > > > > > On 4 Feb 2014, at 18:09, Pascal Egea wrote: > > > Hi Phoebe, > > > > Another possibility is the Emulsiflex (from Avestin, in canada). Not a > cheap piece of equipment, but very sturdy and efficient to up to 200 mL of > extract (runs on house-air). Can deal with E coli (and even yeast if you > have the models with internal compressor). It comes in 3 sizes I think we > have the middle one (C-3) with a compressor. It is basically a french press > but without the inconvenient of being french (I am french myself). It is > quite gentle, and does not overheat samples as much as the sonicator. We > make a lot of membrane protein purifications and I have been working with > this since my post-doc > > Hope this helps. > > > > Best regards, > > > > Pascal Egea > > > > > > On Tue, Feb 4, 2014 at 8:49 AM, Phoebe A. Rice <pr...@uchicago.edu> > wrote: > > Some time ago, there was a nice discussion of cost-effective, wimpy > protein-friendly ways to break open E. coli. We're thinking about > replacing an aging sonicator. If people have a favorite gizmo, could they > repeat that advice? > > thank you, > > Phoebe Rice > > > > ++++++++++++++++++++++++++++++++++++++++++ > > > > Phoebe A. Rice > > Dept. of Biochemistry & Molecular Biology > > The University of Chicago > > > > 773 834 1723; pr...@uchicago.edu > > http://bmb.bsd.uchicago.edu/Faculty_and_Research/ > > > > http://www.rsc.org/shop/books/2008/9780854042722.asp > > > > > > > > -- > > Pascal F. Egea, PhD > > Assistant Professor > > UCLA, David Geffen School of Medicine > > Department of Biological Chemistry > > Boyer Hall room 356 > > 611 Charles E Young Drive East > > Los Angeles CA 90095 > > office (310)-983-3515 > > lab (310)-983-3516 > > email pe...@mednet.ucla.edu > -- Jim Fairman, Ph D. Crystal Core Leader I Emerald Bio <http://www.embios.com> Tel: 206-780-8914 Cell: 240-479-6575 E-mail: fairman....@gmail.com jfair...@embios.com
