Hi Phoebe, Another possibility is the Emulsiflex (from Avestin, in canada). Not a cheap piece of equipment, but very sturdy and efficient to up to 200 mL of extract (runs on house-air). Can deal with E coli (and even yeast if you have the models with internal compressor). It comes in 3 sizes I think we have the middle one (C-3) with a compressor. It is basically a french press but without the inconvenient of being french (I am french myself). It is quite gentle, and does not overheat samples as much as the sonicator. We make a lot of membrane protein purifications and I have been working with this since my post-doc Hope this helps.
Best regards, Pascal Egea On Tue, Feb 4, 2014 at 8:49 AM, Phoebe A. Rice <pr...@uchicago.edu> wrote: > Some time ago, there was a nice discussion of cost-effective, wimpy > protein-friendly ways to break open E. coli. We're thinking about > replacing an aging sonicator. If people have a favorite gizmo, could > they repeat that advice? > thank you, > Phoebe Rice > > ++++++++++++++++++++++++++++++++++++++++++ > > Phoebe A. Rice > Dept. of Biochemistry & Molecular Biology > The University of Chicago > > 773 834 1723; pr...@uchicago.edu > http://bmb.bsd.uchicago.edu/Faculty_and_Research/ > > http://www.rsc.org/shop/books/2008/9780854042722.asp > -- Pascal F. Egea, PhD Assistant Professor UCLA, David Geffen School of Medicine Department of Biological Chemistry Boyer Hall room 356 611 Charles E Young Drive East Los Angeles CA 90095 office (310)-983-3515 lab (310)-983-3516 email pe...@mednet.ucla.edu
