Dear CCP4 community - In case anyone missed this, one interesting example of large conformational changes associated with ligand binding event(s) is T7 RNA polymerase, involved in the initiation, elongation and termination of mRNA synthesis following promoter recognition.
Tom Steitz' review from 2004 [The structural basis of the transition from initiation to elongation phases of transcription, as well as translocation and strand separation, by T7 RNA polymerase. Curr Opin Struct Biol.14(1):4-9] is a good entry point for background information, although there is a large body of work from many biochemists and structural biologists that continues to this day. PDB ID Codes: 'apo'-enzyme: 1aro promoter binary complex: 1cez transcribing complex: 1gln Elongation complex with major conformational change: 1msw Intermediate transcribing complex: 3e3j. This is not meant to be an exhaustive list of what is available in the literature or the PDB, but may whet your curiosity. Wm. Kennedy Sr. Med. Dir. Genenetech On Fri, Feb 28, 2014 at 12:49 AM, Andrew Leslie <[email protected]>wrote: > Dear Jacob, > > Citrate synthase is another early (historically) case, and GAPDH (on > binding NAD). > > Andrew > > On 27 Feb 2014, at 19:43, "Keller, Jacob" <[email protected]> > wrote: > > > Dear Crystallographers, > > > > Does anyone know of good examples of large, reversible conformational > changes occurring between ligand-free and -bound states? Could also be a > non-relevant molecule binding, like sulfate or something inducing dubiously > -relevant changes. I already know of the calmodulin and periplasmic binding > protein families, but does anyone know of others out there? > > > > All the best, > > > > Jacob Keller > > > > ******************************************* > > Jacob Pearson Keller, PhD > > Looger Lab/HHMI Janelia Farms Research Campus > > 19700 Helix Dr, Ashburn, VA 20147 > > email: [email protected] > > ******************************************* >
