Dear Jacob, we published in 2012 the structures of apo and peptide-bound forms of a Dipeptidyl peptidase III:
Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6525-30. doi: 10.1073/pnas.1118005109. Epub 2012 Apr 9. http://www.ncbi.nlm.nih.gov/pubmed/22493238 we observed a mainly rotational motion of approximately 60 degrees between the two lobes of the enzyme upon peptide binding. Regards, Gustavo Dr. Gustavo Arruda Bezerra Group Djinovic Department of Structural and Computational Biology Max F. Perutz Laboratories University of Vienna Campus Vienna Biocenter 5 A-1030 Vienna, Austria http://www.mfpl.ac.at/ Em Quinta-feira, 27 de Fevereiro de 2014 20:43, "Keller, Jacob" <[email protected]> escreveu: Dear Crystallographers, Does anyone know of good examples of large, reversible conformational changes occurring between ligand-free and -bound states? Could also be a non-relevant molecule binding, like sulfate or something inducing dubiously -relevant changes. I already know of the calmodulin and periplasmic binding protein families, but does anyone know of others out there? All the best, Jacob Keller ******************************************* Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Farms Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: [email protected] *******************************************
