Wow, pretty cool—you must have solved it to very high resolution to know the sequence from the structure. I cannot imagine, however, how you got this contaminant—maybe phage infection of your bacterial culture? Anyway, I agree with BLAST-ing the sequence, seeing what you get that is closest.
JPK From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jiyong Su Sent: Thursday, December 14, 2017 7:09 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Crystal structure of an unknown protein Dear CCP4bb, In 2014, I collected a high quality data set from a crystal. But I could not solve the structure of that crystal because this protein is a contaminate. Recently, I used StruBE's Contaminer and fortunately got the solution. Thanks ContaMiner!!! This protein is a contaminate protein. However, I found this protein is an unknown protein (about 180 residues) whose amino acid sequence is not totally same as E.coli. There are about 20 point mutation sites comparing to the E.coli protein. This means this protein may be from an unknown bacteria. The space group of this crystal is new. There is also a new ligand in this protein. My question is how could I found the primary structure of this protein and how to deposit this protein in PDB. Best regards, Jiyong
