I wonder why you assume know there are "about 20 point mutation sites” if "this protein is an unknown protein”. It looks like you are comparing the sequence of a protein you do not know what it is to the sequence of a protein you dont really know what it is (1).
I would consider it more likely, it is the E. coli protein and the electron density is ambigious so some side chains might have been erronously assigned to a false identity. A. (1) Percy: You know, they do say that the Infanta's eyes are more beautiful than the famous Stone of Galveston. Edmund: Mm! ... What? Percy: The famous Stone of Galveston, My Lord. Edmund: And what's that, exactly? Percy: Well, it's a famous blue stone, and it comes ... from Galveston. Edmund: I see. And what about it? Percy: Well, My Lord, the Infanta's eyes are bluer than it, for a start. Edmund: I see. And have you ever seen this stone? Percy: (nods) No, not as such, My Lord, but I know a couple of people who have, and they say it's very very blue indeed. Edmund: And have these people seen the Infanta's eyes? Percy: No, I shouldn't think so, My Lord. Edmund: And neither have you, presumably. Percy: No, My Lord. Edmund: So, what you're telling me, Percy, is that something you have never seen is slightly less blue than something else you have never seen. >> Dear CCP4bb, >> In 2014, I collected a high quality data set from a crystal. But I could not >> solve the structure of that crystal because this protein is a contaminate. >> Recently, I used StruBE's Contaminer and fortunately got the solution. >> Thanks ContaMiner!!! This protein is a contaminate protein. >> However, I found this protein is an unknown protein (about 180 residues) >> whose amino acid sequence is not totally same as E.coli. There are about 20 >> point mutation sites comparing to the E.coli protein. This means this >> protein may be from an unknown bacteria. >> The space group of this crystal is new. There is also a new ligand in this >> protein. >> My question is how could I found the primary structure of this protein and >> how to deposit this protein in PDB. >> Best regards, >> Jiyong > > ----
