Hi Jiyong,
Try running the FITMUNK server in the sequencing mode:
http://witold.med.virginia.edu/fitmunk/server/
More information on using FITMUNK to identify your protein:
https://www.ncbi.nlm.nih.gov/pubmed/26894674
And here:
https://www.ncbi.nlm.nih.gov/pubmed/26660914
Ivan
With best regards,
Ivan Shabalin, Ph.D.
Research Scientist,
Department of Molecular Physiology and Biological Physics,
University of Virginia,
1340 Jefferson Park Avenue, Pinn Hall,Room 4223,
Charlottesville, VA 22908
On 12/14/2017 07:08 AM, Jiyong Su wrote:
Dear CCP4bb,
In 2014, I collected a high quality data set from a crystal. But I could
not solve the structure of that crystal because this protein is a
contaminate.
Recently, I used StruBE's Contaminer and fortunately got the solution.
Thanks ContaMiner!!! This protein is a contaminate protein.
However, I found this protein is an unknown protein (about 180 residues)
whose amino acid sequence is not totally same as E.coli. There are about
20 point mutation sites comparing to the E.coli protein. This means this
protein may be from an unknown bacteria.
The space group of this crystal is new. There is also a new ligand in
this protein.
My question is how could I found the primary structure of this protein
and how to deposit this protein in PDB.
Best regards,
Jiyong
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