Hi Pavel,
Obviously higher resolution typically means a more accurate atomic model of a crystal structure, but I also think that a 1.05 Ang structure is only going to provide you with a great deal more insight than a 1.1 or 1.2 Ang structure in very specific cases. Particularly if your stats aren't completely ideal , why not make sure you get the best possible stats out of your data by slightly cutting the resolution, which is still going to leave you with a VERY high resolution data set (at least I would consider 1.1 or 1.2 Ang as very high resolution)? Please forgive my ignorance, but I have to admit I haven't researched this topic. Is there something wrong with using TLS refinement coupled with anisotropic refinement in Refmac? I just checked and I have found that TLS still provides a small drop in the R factors when comparing anisotropic refinement to TLS + anisotropic refinement in the 1.2 Ang data set I'm currently working on. Ok, it's just under 0.5%, which I admit isn't a huge change, but shouldn't I take any improvement I can get? Cheers, Tony ------------------------------------------------------ Dr. Antonio Ariza University of Oxford Sir William Dunn School of Pathology South Parks Road Oxford OX1 3RE e-mail: antonio.ar...@path.ox.ac.uk<mailto:antonio.ar...@path.ox.ac.uk> Tel: 00 +44 1865 285655 Links to my public profiles: ResearchGate<https://www.researchgate.net/profile/Antonio_Ariza> LinkedIn<https://www.linkedin.com/in/antonioariza1> GoogleScholar<https://scholar.google.co.uk/citations?user=9pAIKV0AAAAJ&hl=en> Twitter<https://twitter.com/DrAntonioAriza?lang=en> Check out my latest paper!!! Structural insights into the function of ZRANB3 in replication stress response<http://www.nature.com/articles/ncomms15847> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
