Hi Sergei, this publication should be useful for you.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756611/
Additionally it is proposed that when your protein is not so stable
(lower Tm), one should incubate the screens at 4C.
http://scripts.iucr.org/cgi-bin/paper?S0907444911036225
Br, Georg.
Am 2019-08-01 um 2:54 PM schrieb Patrick Shaw Stewart:
Hi Sergei
We did some data-mining on this way, way back, in 2004.
See the second section in this link
https://www.douglas.co.uk/PDB_data.htm
When you consider the /non-standard /temps - ie NOT 4C or 20C - it
/looks/ as though the higher-end temps /may /work better. But of
course it's hard to make sense of the results of a martingale.
Thx Patrick
PS Janet (Newman) do you have anything more up-to-date on this?
On Thu, Aug 1, 2019 at 10:24 AM Sergei Strelkov
<sergei.strel...@kuleuven.be <mailto:sergei.strel...@kuleuven.be>> wrote:
Dear all,
I wondered if someone could point me to a recent study on the
importance of temperature during initial search for
crystallization conditions. It would be interesting to see any
real statistics on this subject.
We typically try to perform screening at at two temperatures, such
as duplicating a given kit screen at 20C and 4C if there is enough
sample. My 'gut feeling' is that this is not as important as
sampling the chemical space though.
Thank you!
Sergei
Prof. Sergei V. Strelkov Laboratory for Biocrystallography
Department of Pharmaceutical Sciences, KU Leuven O&N2, Campus
Gasthuisberg, Herestraat 49 bus 822, 3000 Leuven, Belgium Phone:
+32 16 33 08 45, mobile: +32 486 29 41 32 Lab pages:
http://pharm.kuleuven.be/Biocrystallography
<http://pharm.kuleuven.be/anafar>
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