Dear Prem, before moving on with any optimization rounds it would be good to first confirm that these crystals are actually protein crystals, and in particular the protein of interest.
For example, a Silver-stained SDS-PAGE gel can be very informative. You can run a couple of your crystalline clusters after 2 washing steps on SDS-PAGE. Make sure you also load the washing steps, the content of clear drops (from the same screen), and protein as purified,- as controls. We have found that such a simple diagnostic early in the process can be crucially important. best wishes Savvas > On 8 Sep 2019, at 06:39, Prem Prakash <[email protected]> wrote: > > Dear all, > Sorry for a trivial query. I am trying to Co-crystallize my protein with its > substrate (peptide) using commercial screenings. In one condition of JCSG > plus (Molecular Dimension) that contains 0.2 M Magnesium chloride > hexahydrate, 0.1 M Tris 8.5 50 % v/v Ethylene glycol, I got needle like > crystals (picture attached). Does anyone have idea to optimize such needles > into better crystals. I would appreciate all your suggestions. > > Thank you > With kind regards, > Prem Prakash (Ph.D.) > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1><Xtal.jpg> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
