Dear prem, I am sharing my experience with needle crystal that worked for me.
1.First check that salt or a protein crystal by SDS PAGE or mass spectroscopy. 2. For improvement of morphology and size, you could try microseeding of your crushed crystal, generally needle crystal appear quickly, further this could be improved by seeding them again in 96 well plates with commercial screens. If this yield any improved crystals then Pick the best looking and larger sized crystal to seed again into 24 well plates, by streaking the seed solution using cat whisker. 3. If you get any better crystal from seeding, then do a grid screening around the original condition. 4. Try to do different volume ratios of protein with well solution, and even keep them at cold room. Also you could vary the protein concentration. 5. Do an additive screen as well, once you figure out condition that yield better crystal morphology and with improved thickness. Hope this helps Best Rajivgandhi Sundaram Macromolecular crystallography lab institute of life Sciences India. On Sun, Sep 8, 2019, 10:10 AM Prem Prakash <[email protected]> wrote: > Dear all, > Sorry for a trivial query. I am trying to Co-crystallize my protein with > its substrate (peptide) using commercial screenings. In one condition of > JCSG plus (Molecular Dimension) that contains 0.2 M Magnesium chloride > hexahydrate, 0.1 M Tris 8.5 50 % v/v Ethylene glycol, I got needle like > crystals (picture attached). Does anyone have idea to optimize such needles > into better crystals. I would appreciate all your suggestions. > > Thank you > With kind regards, > Prem Prakash (Ph.D.) > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
