4. Matrix microseeding. Make a seed stock from these crystals and then re-run your primary screens.
https://www.ncbi.nlm.nih.gov/m/pubmed/25195878/ -- Dr David C. Briggs Senior Laboratory Research Scientist Signalling and Structural Biology Lab The Francis Crick Institute London, UK == about.me/david_briggs ________________________________ From: CCP4 bulletin board <[email protected]> on behalf of chitra latka <[email protected]> Sent: Sunday, September 8, 2019 12:12:28 PM To: [email protected] <[email protected]> Subject: Re: [ccp4bb] Optimization from needle shaped crystals I can share what has worked for my crystals : 1. You can put a grid across your condition with same or altered drop ratios. 2. You can try micro seeding. (This has given me the best results so far). 3. You can try Hampton's additive screen. On Sun, Sep 8, 2019 at 10:09 AM Prem Prakash <[email protected]<mailto:[email protected]>> wrote: Dear all, Sorry for a trivial query. I am trying to Co-crystallize my protein with its substrate (peptide) using commercial screenings. In one condition of JCSG plus (Molecular Dimension) that contains 0.2 M Magnesium chloride hexahydrate, 0.1 M Tris 8.5 50 % v/v Ethylene glycol, I got needle like crystals (picture attached). Does anyone have idea to optimize such needles into better crystals. I would appreciate all your suggestions. Thank you With kind regards, Prem Prakash (Ph.D.) ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2Fwebadmin%3FSUBED1%3DCCP4BB%26A%3D1&data=02%7C01%7C%7C9e5bd9b3c9e7485ed8af08d7344ee01c%7C4eed7807ebad415aa7a99170947f4eae%7C0%7C1%7C637035385686224007&sdata=635CdZ1OnWv%2BoTW6YrzI%2BJaPg64OrVyWzz7p9HiAt2c%3D&reserved=0> -- Regards Chitra ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2Fwebadmin%3FSUBED1%3DCCP4BB%26A%3D1&data=02%7C01%7C%7C9e5bd9b3c9e7485ed8af08d7344ee01c%7C4eed7807ebad415aa7a99170947f4eae%7C0%7C1%7C637035385686234001&sdata=iT7qhIjkuHu1eO%2BqZMthtr4gWL%2B3pI0eYOfJOR0Jm9I%3D&reserved=0> The Francis Crick Institute Limited is a registered charity in England and Wales no. 1140062 and a company registered in England and Wales no. 06885462, with its registered office at 1 Midland Road London NW1 1AT ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
