Dear Stephen I guess the first thing to understand is whether you have a RT or cryo NMX planned. In my personal experience the transition from RT to cryo NMX is not necessarily straightforward. If you (and the beam line scientist) are sure that cryocooled xtals will give you an experiment then this is the easiest option for an anaerobic sample. However, I would certainly suggest a RT backup.
If you go for RT, given that your xtal will sit in front of neutrons for days I would certainly make sure that once outside your shipping airtight contained your sample is anaerobic for a sufficient length of time. Air diffusion will happen to some extent but it might not be necessarily too bad. That will depend on how sensitive your particular system is to O2. This can also be tested on a X-ray home source after your capillary have been left sitting in air for, let’s say, a week. Best of luck with those deuterons. Roberto On 19 Feb 2020, at 10:22, [email protected]<mailto:[email protected]> <[email protected]<mailto:[email protected]>> wrote: Dear CCP4 community, I have an impending trip to a neutron source and was wondering how people tend to ship their samples prior to beam time? Is sending something frozen best or is sealed in a capillary more sensible or is there another better way? One caveat is that ideally my sample should remain anaerobic which has me leaning towards frozen, but I guess in pcr type tubes sealed in gas tight vessels is also a viable option? Any thoughts or suggestions are very much appreciated. Best wishes, Steve Dr Stephen Carr Research Complex at Harwell (RCaH) Rutherford Appleton Laboratory Harwell Oxford Didcot Oxon OX11 0FA United Kingdom Email [email protected]<mailto:[email protected]> tel 01235 567717 This email and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorized recipient of the addressee, please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to this email. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Research Complex at Harwell. There is no guarantee that this email or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. We use an electronic filing system. Please send electronic versions of documents, unless paper is specifically requested. This email may have a protective marking, for an explanation, please see: https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.mrc.ac.uk%2FAbout%2Finformationandstandards%2Fdocumentmarking%2Findex.htm&data=01%7C01%7Croberto.steiner%40KCL.AC.UK%7C372ff477b0964cdbde1d08d7b525b29b%7C8370cf1416f34c16b83c724071654356%7C0&sdata=MY%2FDvBigM20lZFcAYD9DE864%2FNVeLdi36WiLFrHmwl8%3D&reserved=0. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2Fwebadmin%3FSUBED1%3DCCP4BB%26A%3D1&data=01%7C01%7Croberto.steiner%40KCL.AC.UK%7C372ff477b0964cdbde1d08d7b525b29b%7C8370cf1416f34c16b83c724071654356%7C0&sdata=8izSUcC2WfP6j4zvaBMrtTa8W0T2vSu7N1OkjWgXkT4%3D&reserved=0 Professor Roberto Steiner Randall Centre for Cell and Molecular Biophysics Faculty of Life Sciences and Medicine King's College London [email protected]<mailto:[email protected]> Phone 0044 20 78488216 Fax 0044 20 78486435 Room 3.10A New Hunt's House Guy's Campus SE1 1UL London ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
