Hi Gloria, While we're confessing to minor economies with the truth, I never travelled to the Photon Factory in Japan with crystals of pertussis toxin: they were crystals of Bordetella pertussis ADP-ribosyltransferase.
Randy > On 20 Feb 2020, at 02:47, Gloria Borgstahl <[email protected]> wrote: > > Actually we don't declare anything to TSA, Jahaun just carries them in his > carryon and it is fine. Easy peasy? > > > On Wed, Feb 19, 2020 at 3:48 PM > <[email protected] > <mailto:[email protected]>> wrote: > > ... and don't say you're travelling with heavy water! > > Jon Cooper > > On 19 Feb 2020 17:12, "Azadmanesh, Jahaun" <[email protected] > <mailto:[email protected]>> wrote: > Hello, > > I have traveled to a neutron beamline ~6 times over the past several years. > > I have had awful luck shipping crystals, so I decided to hand-carry and I > found this best. > > I'm not sure what conditions you plan to shoot your crystals. I mount my > crystals in a sturdy quartz capillary (from vitrocom), seal the ends with a > layer of wax, then two coats of nail polish (choose your favorite prettiest > color!) > > I would then wrap the capillary in cotton from a cottonball, then "stuff" the > capillary-cotton combination in a 15 mL falcon tube for a snug fit. For extra > assurance, the falcon tubes were placed inside a generous amount of > bubble-wrap that was then taped inside a small cardboard box. > > I have sought anaerobic conditions for several of my samples to acquire a > desired redox state of my metallo-protein. This was tough, so instead I used > a high-ish concentration of my redox agent dissolved in my reservoir solution > "slugs" in the capillary. These would be replaced in intervals with fresh > slugs prior to the beamtime, and for sure right before the beamtime. The > redox state held for my ~10 days of beamtime and several months after. > > For cryo-conditions, I just placed a bunch of my crystals in a capillary > filled with reservoir solution and sealed as above. I would do my chemical > manipulations and freezing at the beamline. > > Much more details are found in our publication: > https://www.ncbi.nlm.nih.gov/pubmed/30279321 > <https://www.ncbi.nlm.nih.gov/pubmed/30279321> > > Hope this helps! > From: CCP4 bulletin board <[email protected] > <mailto:[email protected]>> on behalf of [email protected] > <mailto:[email protected]> <[email protected] > <mailto:[email protected]>> > Sent: Wednesday, February 19, 2020 4:22 AM > To: [email protected] <mailto:[email protected]> > <[email protected] <mailto:[email protected]>> > Subject: [ccp4bb] Shipping samples for neutron diffraction > > Non-UNMC email > > Dear CCP4 community, > > > I have an impending trip to a neutron source and was wondering how people > tend to ship their samples prior to beam time? Is sending something frozen > best or is sealed in a capillary more sensible or is there another better > way? One caveat is that ideally my sample should remain anaerobic which has > me leaning towards frozen, but I guess in pcr type tubes sealed in gas tight > vessels is also a viable option? > > > Any thoughts or suggestions are very much appreciated. > > > Best wishes, > > > Steve > > > Dr Stephen Carr > Research Complex at Harwell (RCaH) > Rutherford Appleton Laboratory > Harwell Oxford > Didcot > Oxon OX11 0FA > United Kingdom > Email [email protected] <mailto:[email protected]> > tel 01235 567717 > > This email and any attachments may contain confidential, copyright and or > privileged material, and are for the use of the intended addressee only. 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Any unauthorized use or > disclosure of this information is prohibited. If you have received this > e-mail by mistake, please delete it and immediately contact the sender. > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> ------ Randy J. Read Department of Haematology, University of Cambridge Cambridge Institute for Medical Research Tel: + 44 1223 336500 The Keith Peters Building Fax: + 44 1223 336827 Hills Road E-mail: [email protected] Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
