... and don't say you're travelling with heavy water!
Jon Cooper
On 19 Feb 2020 17:12, "Azadmanesh, Jahaun" <[email protected]> wrote:
Hello,
I have traveled to a neutron beamline ~6 times over the past several years.
I have had awful luck shipping crystals, so I decided to hand-carry and I found this best.
I'm not sure what conditions you plan to shoot your crystals. I mount my crystals in a sturdy quartz capillary (from vitrocom), seal the ends with a layer of wax, then two coats of nail polish (choose your favorite prettiest color!)
I would then wrap the capillary in cotton from a cottonball, then "stuff" the capillary-cotton combination in a 15 mL falcon tube for a snug fit. For extra assurance, the falcon tubes were placed inside a generous amount of bubble-wrap that was then taped inside a small cardboard box.
I have sought anaerobic conditions for several of my samples to acquire a desired redox state of my metallo-protein. This was tough, so instead I used a high-ish concentration of my redox agent dissolved in my reservoir solution "slugs" in the capillary. These would be replaced in intervals with fresh slugs prior to the beamtime, and for sure right before the beamtime. The redox state held for my ~10 days of beamtime and several months after.
For cryo-conditions, I just placed a bunch of my crystals in a capillary filled with reservoir solution and sealed as above. I would do my chemical manipulations and freezing at the beamline.
Much more details are found in our publication: https://www.ncbi.nlm.nih.gov/pubmed/30279321
Hope this helps!
From: CCP4 bulletin board <[email protected]> on behalf of [email protected] <[email protected]>
Sent: Wednesday, February 19, 2020 4:22 AM
To: [email protected] <[email protected]>
Subject: [ccp4bb] Shipping samples for neutron diffractionNon-UNMC email
Dear CCP4 community,
I have an impending trip to a neutron source and was wondering how people tend to ship their samples prior to beam time? Is sending something frozen best or is sealed in a capillary more sensible or is there another better way? One caveat is that ideally my sample should remain anaerobic which has me leaning towards frozen, but I guess in pcr type tubes sealed in gas tight vessels is also a viable option?
Any thoughts or suggestions are very much appreciated.
Best wishes,
Steve
Dr Stephen Carr
Research Complex at Harwell (RCaH)
Rutherford Appleton Laboratory
Harwell Oxford
Didcot
Oxon OX11 0FA
United Kingdom
Email [email protected]
tel 01235 567717
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