Hi

I have a weird question. Is it possible to incorporate or mutate amino acid
residues at the interface of two interacting surfaces in a multi subunit
protein complexes hoping it to form a disulfide bond making the over all
complex more stable for downstream structural studies ?

Is there any sort of literature available where creating these types of
modification or kind of protein engineering helped in making a more stable
complex, eventually helping in further structural studies by x-ray or
cryoem ?

Best

kahkashan

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