Hi Firdous, There are several such examples. However, what I wanted to point out is a package that might be of interest, Disulfide by Design. Helps choose residues to mutate to make a disulfide. Made by the Dombkowski lab.
Alexandra Sent from my iPhone > On Mar 16, 2020, at 3:58 AM, Firdous Tarique <[email protected]> > wrote: > > > Hi > > I have a weird question. Is it possible to incorporate or mutate amino acid > residues at the interface of two interacting surfaces in a multi subunit > protein complexes hoping it to form a disulfide bond making the over all > complex more stable for downstream structural studies ? > > Is there any sort of literature available where creating these types of > modification or kind of protein engineering helped in making a more stable > complex, eventually helping in further structural studies by x-ray or cryoem ? > > Best > > kahkashan > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
