Hello everyone Thanks a lot for your valuable feedback. I'll try and let you know.
Best Kahkashan On Mon, Mar 16, 2020 at 2:57 PM Guillaume Gaullier <[email protected]> wrote: > Hi Kahkashan, > > This paper shows a nice example with specific crosslinks in the > nucleosome: https://doi.org/10.1016/j.jmb.2017.10.029 > Of course, having a high-resolution structure helps a lot to design > specific crosslinks like they did… You may not have one if you work on > determining a novel structure. But I hope this paper and references within > will be helpful to you. > > Cheers, > > Guillaume > > > On 16 Mar 2020, at 08:58, Firdous Tarique <[email protected]> > wrote: > > Hi > > I have a weird question. Is it possible to incorporate or mutate amino > acid residues at the interface of two interacting surfaces in a multi > subunit protein complexes hoping it to form a disulfide bond making the > over all complex more stable for downstream structural studies ? > > Is there any sort of literature available where creating these types of > modification or kind of protein engineering helped in making a more stable > complex, eventually helping in further structural studies by x-ray or > cryoem ? > > Best > > kahkashan > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
